Investigation and analysis of indoor radon concentration of urban residents in Shiyan, China / 中国辐射卫生

@#<b>Objective</b> To monitor the indoor radon concentration of urban residents in Shiyan, China, and to analyze the related influencing factors. <b>Methods</b> From April to July, 2019, RSKS standard detectors were used to measure the indoor radon concentration of 125 households in Shiyan, and the results were analyzed. <b>Results</b> The indoor radon concentration of residents in Shiyan showed a skewed distribution, ranging from 13.8 to 145 Bq/m³, and <i>M</i> (<i>P</i>₂₅,<i>P</i>₇₅) was 38.3 (29.0,62.0) Bq/m³. The estimated annual effective dose of radon and radon daughters from inhalation was 0.52-5.50 mSv, and <i>M</i> (<i>P</i>₂₅,<i>P</i>₇₅) was 1.45 (1.10, 2.36) mSv, which was consistent with literature. Building structure (<i>H</i> = 14.10, <i>P</i> < 0.001), floor (<i>H</i> = 24.41, <i>P</i> < 0.001), and geographical region (<i>H</i> = 8.963, <i>P</i> < 0.05) were influencing factors of indoor radon concentration, and the differences were significant. <b>Conclusion</b> The indoor radon concentration of urban residents in Shiyan is lower than the national standard limit. However, in daily life, it is still necessary to take appropriate measures to reduce the concentration of indoor radon as much as possible.

Analysis of noise hazard characteristics in automobile sand casting industry / 公共卫生与预防医学

Objective To study the characteristics of noise hazards in automobile sand casting industry and understand the impact of noise on the health of automobile foundry workers, and to provide scientific data support for the control of noise hazards. Methods An automobile sand casting enterprise was selected as the research object. The post noise intensity test results from 2010 to 2019, the noise spectrum test results of each post in 2019, and the prevalence of occupational noise deafness in the 10 years from 2010 to 2019 were analyzed and sorted out. Using chi-square test and t-test the noise hazard characteristics and noise intensity changes were statistically analyzed. Results 1. The noise LEX.8h exceeding standard rate of foundry enterprises in the 10 years from 2010 to 2019 was not statistically significant（χ2=6.76, P=0.66), and the mean value of noise LEX.8h fluctuated horizontally in the 10 years, which was basically stable between 80 - 90 dB (A). 2. The mean value of noise intensity of sand falling, sand preparation, cleaning and shot blasting in the first five years was significantly different compared to that in the last five years. There was no statistically significant difference in other posts. 3. Among all posts in the foundry sand casting industry, the noise spectrum of core-making and cleaning posts was mainly medium and high frequency, while the noise spectrum of other posts was mainly medium and low frequency. 4, Cases of occupational noise deafness were mainly concentrated in cleaning and polishing workers, melting chemical workers, inspectors and other types of workers. Conclusions The noise hazards in the automobile foundry sand casting industry are mainly concentrated on the posts of molding, and sand falling, and the noise intensity and the over standard rate remain high in the past 10 years. The noise of each post is mainly medium and low frequency, and the noise of a few individual posts is mainly medium and high frequency. In recent years, due to the automatic renewal of equipment, the noise intensity of some posts such as sand dropping, sand mixing, cleaning and shot blasting has decreased, but the overall noise exceeding standard rate has basically not changed, and the noise hazard is still serious. The noise hazard of foundry sand casting enterprises is difficult to be controlled, and the risk of hearing loss of foundry workers is still high.

Clinicopathological features in 507 patients with type 2 diabetes mellitus complicated with chronic kidney disease / 中华肾脏病杂志

Objective:To analyze the clinicopathological features in diabetic kidney disease (DKD) and non-diabetic kidney disease (NDKD) patients, and provide reference for patients who will receive renal biopsy with diabetes mellitus complicated with chronic kidney disease.Methods:The patients with type 2 diabetes mellitus complicated with chronic kidney disease who underwent renal biopsy were collected through the database at the Nanfang Hospital of Southern Medical University from February 2002 to June 2018. According to the results of renal biopsy, they were divided into DKD group and NDKD group (including DKD+NDKD). The clinical manifestations and pathological types were compared between the two groups.Results:A total of 507 patients were eventually included in the study. There were 114 cases (22.5%) with DKD and 393 cases (77.5%) with NDKD. Pathologically, the most common pathological types of NDKD were membranous nephropathy (30.0%) and IgA nephropathy (19.1%). Among NDKD patients, 5.6% patients had DKD combing with NDKD. In term of the clinical manifestations, DKD patients had a longer history of diabetes (>1 year, 76.3% vs 36.1%, P<0.001), higher quantity of urinary protein [3.69(1.70, 6.74) g/24 h vs 2.21(0.91, 4.97) g/24 h, P<0.001], higher serum creatinine [117.5(85.8, 194.5) μmol/L vs 89.0(68.0, 143.8) μmol/L, P<0.001] than NDKD patients. But the hemoglobin [(105.07±20.85) g/L vs (124.41±25.02) g/L, P=0.002] and cholesterol [(5.69±1.87) mmol/L vs (6.43±2.75) mmol/L, P=0.001] in DKD patients were lower than those in NDKD patients. Logistic regression analysis showed that diabetes mellitus history ( OR=4.162, 95% CI 1.717-10.098, P=0.002) , higer systolic pressure (every 1 mmHg, OR=1.028, 95% CI 1.011-1.045, P=0.001) , history of antihypertensive medication ( OR=3.141, 95% CI 1.496-6.591, P=0.002), diabetic retinopathy ( OR=5.561, 95% CI 2.361-13.100, P<0.001) and higher glycated hemoglobin level (every 1%, OR=1.680, 95% CI 1.333-2.118, P<0.001) were related factors of DKD, while hematuria ( OR=2.781, 95% CI 1.334-5.798, P=0.006) and higher hemoglobin level (every 1 g/L, OR=1.022, 95% CI 1.008-1.037, P=0.002) were related factors of NDKD. Conclusions:There are differences in clinical manifestations and pathological types between DKD and NDKD. The history of diabetes, antihypertensive medication, fundus examination, higher of proteinuria and glycosylated hemoglobin may predict DKD, while hematuria and higher level of hemoglobin may have certain guiding significance for the diagnosis of NDKD. The indication of renal biopsy in patients with diabetes mellitus complicated with chronic kidney disease should include comprehensive clinical manifestations.

Energy metabolism changes of renal cortex in a rat model of progressive kidney disease and its mechanisms / 中华肾脏病杂志

Objective To explore the changes of renal cortical energy metabolism and its related molecular mechanisms in rats with progressive kidney disease.Methods A rat model of 5/6 nephrectomy was established as the model of progressive nephropathy.Rats were divided into surgical group (5/6Nx group) and sham-operated group (Sham group).Respectively,the rats were sacrificed at 1 week and 12 weeks after completing the model,and their blood,urine sample and kidney specimens were collected.Blood urea nitrogen,serum creatinine and 24 h urine protein were used to evaluate the renal function.Pathological changes in renal tissue were detected by PAS staining and Sirius red staining.The renal cortical energy metabolites were made quantitative analysis by liquid chromatography-mass spectrometry-based targeted metabolomics.The mRNA expressions of inflammatory cytokines (IL-6,IL-1β),fibrosis factors (fibronectin,collagen-1),glycolytic and tricarboxylic acid (TCA) cycle related enzymes were confirmed by real-time PCR.The protein expressions of fibrotic proteins (fibronectin,collagen-1),silent information regulator 1 (SIRT-1) and liver kinase B1 (LKB1) were tested by Western blotting.Results Compared with those in Sham group,the renal function indexes increased,the renal tissue pathological damage was obvious,the mRNA expressions of renal cortical inflammatory and fibrosis factors increased,and fibrotic proteins also increased in 5/6Nx group rats at 1 week and 12 weeks (all P ＜ 0.05),meanwhile,kidney damage worsened over time.Compared with those in Sham group,in the renal cortex of 5/6Nx group glycolytic metabolite lactate,the TCA cycle metabolites (citrate,isocitrate,oxaloacetate) and the oxidized phosphorylation metabolite reduced coenzyme Ⅰ were up-regulated (all P ＜ 0.05),but adenosine triphosphate (ATP) was no change at 1 week,then the abnormal metabolites increased further at 12 weeks,such as the down-regulation of pyruvate,oxidized coenzyme Ⅰ and ATP (all P ＜ 0.05).The pentose phosphate pathway metabolites (reduction and oxidized coenzyme Ⅱ) shows no statistical significant difference in the two group (all P ＞ 0.05).Compared with those in Sham group,in the 5/6Nx group the mRNA expressions of glycolytic enzyme hexokinase 2 and lactate dehydrogenase a were upregulated in the renal cortex at 1 week,whereas the mRNA expressions of pyruvate dehydrogenase α,pyruvate dehydrogenase β and succinate dehydrogenase of the TCA cycle related enzymes were downregulated (all P ＜ 0.05).Meanwhile,renal abnormal metabolic enzyme mRNA expressions were further increased in the 5/6Nx group at 12 weeks.The protein levels of SIRT-1 and LKB1 were not significantly different in the renal cortex of two group rats at 1 week,while SIRT-1 and LKB1 levels decreased in 5/6Nx group than those in Sham group at 12 weeks (all P＜ 0.05).Conclusions During the progression of nephropathy,rats accompanied with renal fibrosis and inflammatory have energy metabolism changes in the renal cortex which accompanies.The features of metabolic changes are manifested as enhanced glycolysis and decreased oxidative phosphorylation,which is aggravated gradually.Its mechanism is related to the inhibition of energy-regulating proteins LKB1 and SIRT-1.

A comparative study on the construction of medical teams in public primary and secondary schools after multi-channel distribution mode in Guangzhou / 中国学校卫生

Objective@#To understand the situation of school medical staff in Guangzhou after adopting multichannel medical model, and to provide reference for school medical team construction.@*Methods@#A total of 1 099 primary and secondary schools in Guangzhou participated in this study through questionnaire survey regarding school medical personnel, basic situation of school medical personnel and work situation.@*Results@#Among the 1 099 primary and secondary schools surveyed, 638(58.05%) of them consisted of 707 full-time school doctors, the eligible rate was 28.20%. The recruitment of school doctors was still based on independent recruitment(55.87%), and 44.13% of the purchase service modes (community deployment, hospital purchase services, and school clinic custody) were deployed. Most of school medical professionals majored in nursing(56.86%), followed by clinical medicine (26.03%) and preventive medicine (9.76%); In terms of professional titles, junior, intermediate and senior certificates accounted for 52.47%, 37.34%, and 1.27%, respectively; More than 68.74% of school doctors worked for less than 5 years; On average, 68.60% of training were provided every six months. About 33.95% of the school doctor were satisfied with salary, 20.79% were not satisfied. Compared with the year 2016, no significant changes were noticed in academic qualifications, professional titles, and working conditions of school medical team, however, increased proportion of staffs with nursing certificate instead of clinical medicine certificate increased, workload and satisfaction towards salary decreased(P<0.01).@*Conclusion@#After adopting the multi-channel deployment model, the construction of school health workforce in Guangzhou has made breakthrough progress. It is proposed to improve the guidelines for the standardization of school doctors, to strengthen the professional skills training of school doctors, and to better improve the overall level of school health work in the city.

Effects of microwave exposure on the enzymatic activity of Trichophyton rubrum / 中华物理医学与康复杂志

Objective@#To investigate the effects of exposing Trichophyton rubrum fungus to microwaves at different intensities in terms of the activity of succinic dehydrogenase and beta-(1, 3)-D-glucan synthase.@*Methods@#Trichophyton rubrum organisms were randomly divided into a control group and experimental groups. The experimental groups were incubated at 27 ℃ after direct radiation with 2450 MHz microwaves at 20, 40, 60 or 80 W for 15 min, repeated 8 times. The control group was incubated without any irradiation. Thirty days later, the beta-(1, 3)-D-glucan synthase and succinate dehydrogenase activities were determined using enzyme-linked immunosorbent assays.@*Results@#The enzymatic activity decreased gradually with increasing radiation intensity. When the output power was 80 W, the beta-(1, 3)-glucan-synthase-D activity was 0.730±0.74 U/ml and that of the succinate dehydrogenase was 1.828±1.774 U/L, both significantly lower than in the groups subjected to less powerful irradiation.@*Conclusions@#Microwave radiation can decrease the enzymatic activity of Trichophyton rubrum in a dose-dependent manner. Higher intensity is more effective. Microwave irradiation can decrease the activity of succinate dehydrogenase and beta-(1, 3)-glucan synthase from Trichophyton rubrum in vitro, resulting in the destruction of fungal cell walls and interfering with the tricarboxylic acid cycle, furthering cell death. Moreover, the temperature change possibly also helps promote the biological effects of microwave radiation.

Effects of microwave exposure on the enzymatic activity of Trichophyton rubrum / 中华物理医学与康复杂志

Objective To investigate the effects of exposing Trichophyton rubrum fungus to microwaves at different intensities in terms of the activity of succinic dehydrogenase and beta-( 1, 3)-D-glucan synthase. Methods Trichophyton rubrum organisms were randomly divided into a control group and experimental groups. The experimental groups were incubated at 27 ℃ after direct radiation with 2450 MHz microwaves at 20, 40, 60 or 80 W for 15 min, repeated 8 times. The control group was incubated without any irradiation. Thirty days later, the beta-( 1, 3)-D-glu-can synthase and succinate dehydrogenase activities were determined using enzyme-linked immunosorbent assays. Results The enzymatic activity decreased gradually with increasing radiation intensity. When the output power was 80 W, the beta-(1,3)-glucan-synthase-D activity was 0.730±0.74 U/ml and that of the succinate dehydrogenase was 1.828±1.774 U/L, both significantly lower than in the groups subjected to less powerful irradiation. Conclusions Microwave radiation can decrease the enzymatic activity of Trichophyton rubrum in a dose-dependent manner. Higher intensity is more effective. Microwave irradiation can decrease the activity of succinate dehydrogenase and beta-( 1, 3)-glucan synthase from Trichophyton rubrum in vitro, resulting in the destruction of fungal cell walls and interfering with the tricarboxylic acid cycle, furthering cell death. Moreover, the temperature change possibly also helps promote the biological effects of microwave radiation.

Distribution of Astrocytic Syntrophin in Hippocampus from Human Mesial Temporal Lobe Epilepsy / 中国康复理论与实践

Objective To investigate the expression changes of astrocytic syntrophin in hippocampus from human mesial temporal lobe epilepsy (MTLE). Methods From April, 2015 to July, 2016, 17 cases of hippocampus, collected from temporal lobectomy, were divided into MTLE group (n=13) and non-MTLE group (n=4) according to hematoxylin and eosin staining, glial fibrillary acidic protein and neuronal nu-clei immunohistochemical staining. Immunofluorescence double labeling and immunofluorescence histochemistry were used to observe the expression of syntrophin. Results The proliferation of astrocytes increased and neurons reduced in the hippocampus of MTLE group. Syntro-phin was found in the membrane and foot processes of astrocyte, that was enriched along perivascular astrocyte end-feet domain in non-MTLE group, but lost in MTLE group. While the whole expression of syntrophin was more in MTLE group than in non-MTLE group (t=5.421, P<0.001). Conclusion The distribution of syntrophin in hippocampus astrocytes may be related to the development of MTLE.

Expression of Aquaporin 4 in Astrocytes of Rats in Vitro / 中国康复理论与实践

Objective To explore the expression of aquaporin 4 (AQP4) in primary and secondary cultured rat astrocytes. Methods Rat cortical astrocytes from a newborn (one day) Wistar rat were cultured. Astrocytes were identified with immunofluorescence staining of glial fibrilillary acidic protein (GFAP). The expression of AQP4 was determined with real-time quantitative polymerase chain reaction and immu-nofluorescence staining three, five, seven and nine days of primary culture, and nine days of secondary culture. Results The purity of GFAP-positive cells was more than 95％. The expression of AQP4 mRNA was found three days of primary culture, remained unchanged five days of primary culture (P>0.05), and increased seven and nine days of primary culture (P0.05). AQP4 immunofluorescence staining showed the same trend of AQP4 mRNA. Conclusion AQP4 may express since three days of primary culture in rat astrocytes in vitro, and increase slowly until nine days of primary culture.

Lentivirus Delivery of the Short Hairpin RNA Targeting NDV P Gene Inhibits Production of the Newcastle Disease Virus in Chicken Embryo Fibroblasts and Chicken Embryos / 病毒学报

Small interfering ribonucleic acid (siRNA)-induced RNA degradation can inhibit viral infection, and has been investigated extensively for its efficacy as antiviral therapy. The potential therapeutic role of lentiviral-mediated short hairpin ribonucleic acid (shRNA) to Newcastle disease virus (NDV) replication in vivo has been explored less often. We constructed two recombinant lentiviral vectors containing shRNA against the phosphoprotein (P) of the NDV, RNAi-341 and RNAi-671. Recombinant shRNA lentivirus vectors were co-transfected into 293T cells, along with helper plasmids, to package the recombinant shRNA lentivirus. Lentivirus-based shRNAs were titrated and transduced into NDV-susceptible chicken embryo fibroblasts (CEFs) and chick embryos. Antiviral activity against the NDV strain was evaluated by virus titration and real-time reverse transcription-polymerase chain reaction. RNAi-341 and RNAi-671 strongly suppressed transient expression of a FLAG-tagged P fusion protein in 293T cells. RNAi-341 and RNAi-671 NDV reduced virus titers by 66.6-fold and 30.6-fold, respectively, in CEFs 16 h after infection. RNAi-341 and RNAi-671 reduced virus titers in specific pathogen-free chick embryos by 99% and 98%, respectively, 48 h after infection. Both shRNAs inhibited accumulation of not only P-gene mRNA, but also nucleocapsid, M-, F-, HN-, and L-gene mRNA. RNAi-341 silenced P-gene mRNA more potently than RNAi-671. These results suggest that shRNAs silencing the P gene had substantial antiviral properties and inhibited NDV replication in CEFs and chick embryos.

Comparison of the differences in glutamate-induced astrocyte swelling between Wistar and Sprague-Dawley rats / 中国实验动物学报

Objective To compare the differences between the cell swelling of cultured astrocytes ( AST) from Wistar and Sprague-Dawley ( SD) rats after incubation with glutamate.Methods Primary cultured AST derived from the cerebral cortex of one-day-old Wistar or SD rats were prepared.The cultured AST received 1 or 10 mmol/L glutamate treat-ment for 48 h on the tenth day after subculture.The viability of AST was determined by lactate dehydrogenase ( LDH) kit to assess the cell injury, and the perimeter of AST was measured using Image Pro Plus software after glial fibrillary acidic protein immunofluorescence staining to evaluate the astrocyte swelling.Then, the expression of aquaporin 4 ( AQP4 ) in cultured AST was detected by quantitative reverse transcription polymerase chain reaction.Results No significant differ-ence was found in the LDH release after the glutamate treatment in cultured AST from these two strains (P>0.05).The perimeter of AST from normal Wistar rats was shorter than that from SD rats, but was longer after the treatment of glutamate (P<0.05).Meanwhile, AQP4 expression in the Wistar rats was significantly higher than that from SD rats after incuba-tion with 1 mmol/L glutamate ( P<0.05 ) .Conclusions These results suggeste that cultured AST from Wistar rats are more susceptible to glutamate-induced swelling than that from SD rats, and there are differences between the effects of glu-tamate on AQP4 expression in astrocytes of Wistar and SD rats.

Effect of Methylene Blue on Blood-brain Barrier after Focal Cerebral Ischemia-reperfusion in Rats / 中国康复理论与实践

Objective To investigate the protective effect of methylene blue (MB) on blood-brain barrier (BBB) injury after focal cere-bral ischemia-reperfusion in rats. Methods 18 male Sprague-Dawley rats were randomly divided into sham-operated group (n=6), model group (n=6) and MB treatment group (n=6). The left middle cerebral arteries were occluded for 1 hour and reperfused. MB was infused intra-venously immediately after reperfusion (3 mg/kg) and again 2 hours post-reperfusion (1.5 mg/kg), while normal saline was administered in the model group. The sham-operated group was treated as same as the model group without occlusion and infusion. HE staining was used to observe the histological injury in the cortex around the infarcted region 47 hours after reperfusion, while albumin immunohistochemistry was used to evaluate the permeability of the BBB, and immunohistochemistry and double immunofluorescence staining were used to exam-ine the expressions of glial fibrillary acidic protein (GFAP) and aquaporin-4 (AQP-4). Results HE staining showed that cells and blood ves-sels were not intact in the cortex around the infarcted region in the model group and they were better in the MB treatment group. The expres-sions of the albumin, GFAP and AQP-4 were higher in the model group than in the sham-operated group (P<0.01), and were lower in MB treatment group than in the model group (P<0.05). The double immunofluorescence staining showed the colocalization of GFAP and AQP-4 in the astrocytes. Conclusion MB may ameliorate the BBB disruption induced by focal cerebral ischemia-reperfusion through reducing glio-cyte proliferation and down-regulation of AQP-4 expression in rats.

The comparison of different quantitative criteria of the Dawn Phenomenon and its impact on blood glucose fluctuation in type 2 diabetes / 中华内分泌代谢杂志

[Summary] A total of 128 individuals with type 2 diabetes underwent continuous glucose monitoring for 3 consecutive days.The dawn phenomenon was defined by three different parameters according to the previous research:(1)the absolute increase of glucose level from nocturnal nadir to prebreakfast value(?G) above 20 mg/dl;(2)?G above 10 mg/dl;( 3 ) insulin requirement increased at least 20%.The participants were secondarily separated by presence/absence of a dawn phenomenon based on the definitions above.The impact on blood glucose fluctuation of different groups was assessed according to the standard deviation of blood glucose( SDBG) , the area under curve above 10 mmol/L ( AUC ) , and the mean amplitude of glycemic excursions ( MAGE ) , etc.The frequencies of dawn phenomenon were 64.8%(?G≥20mg/dl), 85.2%(?G≥10 mg/dl), and 59.4%(rise in insulin requirement≥20%)respectively.The impacts on SDBG, AUC, MAGE, and MODD were without statistical difference(P>0.05) between the presence and absence of the dawn phenomenon patients when?G≥10 mg/dl.However, the differences reached statistical significance(P<0.05) when ?G≥20 mg/dl and the increase in insulin requirement≥20%. Besides, the incidence of dawn phenomenon was positively correlated with HOMA-IR, HbA1C , and free C-peptide.Dawn phenomenon is a very frequent event in type 2 diabetes and not only impacts the overall glycemic control but also exaggerates glucose fluctuation.To be clinically relevant, ?G≥20mg/dl should be taken as the quantitative criterion of the dawn phenomenon.

Study on comparation of three methods for anti-double-stranded DNA antibody and diagnostic value of joint detection in systemic lupus erythematosus / 国际检验医学杂志

Objective To analyse the differences of indirect immuno‐fluorescence (IIF) ,enzyme‐linked immunosorbent assay (ELISA) and immunoblotting technique(IBT) for the determination of anti‐dsDNA antibody ,and evaluate the value of joint detec‐tion of the three methods for diagnosing systemic lupus erythematosus(SLE) .Methods From January 2012 to March 2015 ,50 ca‐ses of patients with SLE ,100 cases of patients with other autoimmune disease(AID)and 100 healthy individuals were selected .Ser‐um levels of anti‐dsDNA antibody were detected by using IIF ,ELISA and IBT respectively .Then ,compared the sensitivity and spe‐cificity of the three methods ,and analysed the sensitivity and specificity of joint detection .Results The IIF method had the highest specificity(99 .5% ) ,while ELISA had the highest sensitivity(74 .0% ) .There were statistically significant differences in the positive detection rates of serum anti‐dsDNA antibody in patients with SLE between IIF and ELISA ,IIF and IRT ,ELISA and IBT(χ2 values were 11 .435 ,13 .994 and 4 .539 ;P<0 .05) ,and the Kappa values were 0 .411 ,0 .522 and 0 .278 respectively .The specificity of three methods joint in series was increased to 99 .5% ,and the sensitivity of parallel combined detection of the three methods was in‐creased to 82 .0% .Conclusion Among the three methods for detecting anti‐dsDNA antibody ,ELISA has the highest sensitivity , and IIF has the highest specificity .Moreover ,joint detection could increase the sensitivity and specificity .

Study on correlation between serum HCV-RNA level and Anti-HCV with liver fibrosis indexes in patients with chronic hepatitis C / 国际检验医学杂志

Objective To investigate the correlation between serum HCV-RNA level and anti-HCV with liver fibrosis indexes in the patients with chronic hepatitis C.Methods The serum samples were collected in 96 cases of chronic hepatitis C.The HCV-RNA level was determined by real time fluorescence quantification PCR and Anti-HCV was detected by ELISA.LN,PCⅢ and CⅣlevels were detected by the electrochemical iuminescence analyzer and HA was examined by radioimmunoassay(RIA).The correla-tion between the HCV-RNA and Anti-HCV levels with the liver fibrosis indexes was analyzed with the statistic software.Results Among these 96 cases of Anti-HCV positive chronic hepatitis C,52 cases (54.2%)were HCV-RNA positive.With increasing of the S/CO value of Anti-HCV,the detection rate of HCV-RNA was increased,which were 9.5%,37.1% and 92.5%.The differ-ences in HA,LN,PCⅢ and CⅣ levels between the high viral load group and the low viral load group had no statistical significance (P >0.05).Conclusion The positive detection rate of HCV-RNA is related with the S/CO value of Anti-HCV,the higher the S/CO value,the higher the positive detection rate of HCV-RNA.But the HCV-RNA level has no correlation with the liver fibrosis in-dexes.

Correlation analysis between nature of carotid plaque by dual-source CT examination and serum levels of hypersensitive C-reactive protein, lipoprotein a and homocysteine / 中国医师进修杂志

Objective To discuss the correlation between nature of carotid plaque by dual-source CT examination and serum levels of hypersensitive C-reactive protein (hs-CRP),lipoprotein a (LPa) and homocysteine (Hcy),and provide a better theoretical basis for the prevention and treatment of ischemic cerebrovascular disease.Methods Fifty carotid plaque patients with ischemic cerebrovascular disease symptoms (group A),50 carotid plaque patients without ischemic cerebrovascular disease symptoms (group B)and 50 healthy people (group C) were selected.All objects were detected the carotid plaque by dual-source CT,and the serum levels of hs-CRP,LPa and Hcy were detected by the automatic biochemical analyzer.The correlation between the nature of carotid plaque by dual-source CT examination and the serum levels of hs-CRP,LPa and Hcy was analyzed.Results The total number of carotid plaque,number of unstable plaque and number of stable plaque in group A were 357,220 and 137 pieces,respectively.Those in group B were 200,13 and 187 pieces,respectively.There were statistical differences between group A and group B (P ＜ 0.01).Patients in group A and group B were mainly with unstable plaque and stable plaque,respectively.Healthy people in group C were without carotid plaque.The serum levels of hs-CRP,LPa and Hcy in group A were (36.74 ± 13.22) mg/L,(563.15 ± 178.69) mg/L and (40.15 ± 12.31) μ mol/L,respectively.These in group B were (12.36 ± 4.35) mg/L,(393.56 ± 86.45) mg/L and (29.45 ± 7.63) μ mol/L,respectively.These in group C were (3.26 ± 1.26) mg/L,(220.17 ± 35.24) mg/L and (14.32 ± 2.44) μ mol/L,respectively.The serum levels of hs-CRP,LPa and Hcy in group A were significantly higher than those in group B and group C,and those in group B were significantly higher than those in group C,there were statistical differences (P ＜ 0.05).The serum levels of hs-CRP,LPa and Hcy were positive correlation with the number of carotid unstable plaque,the correlation coefficients between the serum levels of hs-CRP,LPa and Hcy and the number of carotid unstable plaque in group A were 0.469,0.398 and 0.363 (P ＜ 0.05),respectively,in group B were 0.276,0.145 and 0.127(P ＜ 0.05),respectively.The correlation coefficients between group A and group B were significantly different (P ＜ 0.05).Conclusions The combination of dual-source CT examination and the serum levels of hs-CRP,LPa and Hcy is more beneficial to judge the stability of plaque,and it can help predict the nature of the carotid plaque.This view is important clinically for the prevention and treatment of the occurrence and the development of the ischemic cerebrovascular disease.

The curative effect analysis of oralYiyuan-Guchangdecoctionand enema retention of Yuyangyein treating chronic ulcerative colitis / 国际中医中药杂志

Objective To research the efficacy of oralYiyuan-Guchangdecoction and enema retention ofYuyangye in treating chronic ulcerative colitis(CUC).Methods 70 patients of CUC were randomly divided into two groups: a control group of 34 patients, treated by mesalazine(etiasa) orally, 1g/twice daily; and a test group of 36 patients, treated byYuyangyedecoction for oral administration andYuyangyeenema retention. The treatment course of both groups was 8 weeks. The curative effect, clinical symptoms, endoscopic sign and pathology, and ESR, CRP, IL-8, and IL-13 after one course of treatment, were analyzed statistically.Results The total effect of the test group and the control group was 94.44%(34/36) and 64.71%(22/34), and the difference was significant statistically(Z=-4.207,P0.05)between the two groups, while IL-13 of the test group was superior than the control group(P<0.05).Conclusion The therapeutic effect of oralYiyuan-Guchangdecoction and Yuyangyeenema retention is good in treating chronic ulcerative colitis.

Expression of matrix aggrecanase 2 and a tissue inhibitor of metalloproteinases in degenerated intervertebral discs / 中华物理医学与康复杂志

Objective To observe the expression of aggrecanase 2 and a tissue inhibitor of metalloproteinase 3 (TIMP-3) in degenerate human lumbar intervertebral discs and their role in degeneration of the nucleus pulposus.Methods Pfirrmann classification was used to class degenerate intervertebral discs observed through MRI.They were divided into three groups:a control group (Pfirrmann grade Ⅰ-Ⅱ),a degeneration group (Pfirrmann grade Ⅲ-Ⅳ),and a severe degeneration group (Pfirrmann grade Ⅴ).A total of 45 cases accepted lumbar spine surgery for removing nucleus pulposus specimens.Each group contained 15 cases.After formalin-fixation and paraffin embedding,immunohistochemistry was used to detect aggrecanase 2 and TIMP-3 expression in the nucleus pulposus cells.Results The percentages of cells positive for aggrecanase 2 were (13.58 ± 7.76) ％,(33.48 ± 13.95) ％ and (56.00 ± 18.39) ％ in the control,degeneration and severe degeneration groups respectively.These differences had statistical significance.The percentages of cells positive for TIMP-3 were (34.78 ± 13.80) ％,(46.77 ± 10.98) ％ and (50.65 ± 16.45) ％,and these differences were again statistically significant.The aggrecanase 2/TIMP-3 ratios were also significantly different.Conclusion As the degree of degeneration of the nucleus pulposus increased,the expression of aggrecanase 2 and TIMP-3 rose,which indicates that both changes were closely connected with the degeneration.Their ratio was correlated with the degree of degeneration of the nucleus pulposus.

Effect of Silver Needle Thermo-conduction on Cervical Spondylosis of Vertebral Artery Type / 中国康复理论与实践

@#Objective To explore the effect of silver needle thermo-conduction on cervical spondylosis of vertebral artery type (CSA). Methods 82 patients with CSA were randomly divided into control group (n=41) and treatment group (n=41). The control group was treated with cervical traction combined with microwave diathermy, and the treatment group was treated with silver needle thermo-conduction combined with cervical traction. Both groups were evaluated with Evaluation Scale for Cervical Vertigo (ESCV) and transcranial Doppler before and 1 course after treatment. Results The ESCV score, the peak blood flow velocity of period of contraction of left vertebral artery, right vertebral artery and basilar artery in both groups were significantly higher after treatment than before (P<0.01), the treatment group was better than the control group after treatment (P<0.01). Conclusion Silver needle thermo-conduction combined with cervical traction therapy can accelerate the blood flow of patients with CSA and improve their clinical symptoms

Sensitivity of Human Glioblastoma Multiforme Cell Line BT325 to Antineoplastic Drugs / 中国康复理论与实践

@#Objective To examine the sensitivity of human glioblastoma multiforme cell line BT325 to 5 antineoplastic drugs, including cisplatin (DDP), teniposide (VM26), nimustine (ACNU), temozolomide (TMZ) and vincristine (VCR). Methods BT325 cells were incubated in DMEM with 10% or 20% fetal bovine serum (FBS) or without FBS respectively. The cell numbers were counted at 24 h, 48 h, 72 h,96 h, 120 h, and 144 h, then platting and growth curve were drafted. Cell counting kite-8 was used to detect the influence of 5 drugs with different concentrations on human glioma cell line BT325. Results DDP and VM26 significantly suppressed BT325 cells(>75%) viability in a dose-dependent manner, while VCR inhibited BT325 cells (50%) growth without dose-effect relationship. In contrast, ACNU and TMZwere not effective on the viability of BT325 cells. Conclusion BT325 cells were very sensitive to chemotherapeutic drugs DDP amd VM26.